arf6 influences tumor cell proliferation  (Cytoskeleton Inc)

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Expression levels and prognostic significance of ARF6 in various cancer types. ( A ) Expression levels of ARF6 across 30 distinct types of cancer based on TCGA and GTEx data. ( B ) Prognostic significance of ARF6 in 8 distinct types of cancer-based on TCGA data. ACC: adrenocortical carcinoma, BLCA: bladder urothelial carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, DLBC: diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, AML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SKCM: skin cutaneous melanoma, STAD: stomach adenocarcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Expression of ARF6 in acute myeloid leukemia (AML) patient and its cell lines. ( A ) Differential expression of ARF6 in GSE9476 in AML ( n = 38) and normal controls ( n = 26). ( B ) Expression of ARF6 relative to β-actin was detected in clinical samples including healthy subjects ( n = 3) and AML( n = 4) using RT-qPCR. ( C ) Receiver operating characteristic (ROC) analysis of ARF6 in AML. ( D ) Expression of ARF6 based on CCLE in 55 tumor cell lines. ( E ) Expression of ARF6 in 43 AML cell lines. ( F ) Expression of ARF6 relative to β-actin was detected in U937, THP-1, MV-4 -11, and BMNCs of healthy donors. ** p < 0.01, *** p < 0.001, **** p < 0.0001

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing, Quantitative Proteomics, Quantitative RT-PCR

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Correlation between ARF6 expression and clinical parameters among patients with AML. ( A ) Association of ARF6 expression with age, cytogenetics, and survival status in AML patients. Rectangles represent different variables and their widths usually indicate the size of the number of patients in that state, the larger the width, the greater the number of patients in that state. Bands connect different rectangular nodes, the width of which reflects the relative size of this flow, the wider the width, the greater the number of patients. ( B ) Relationship between white blood cell (WBC) counts and ARF6 expression. ( C ) ARF6 expression in different French-American-British (FAB) classifications of AML. ( D ) Expression of ARF6 in bone marrow (BM) blasts of AML patients. ( E ) Expression of ARF6 in different genders of AML patients. * p < 0.05, ** p < 0.01, “ns” indicates not significant

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Prognostic value of ARF6 expression in AML subgroups. ( A - F ) Prognostic significance of ARF6 expression in various AML subgroups, including ( A ) patients older than 60 years. ( B ) patients with peripheral blood WBC counts less than 20 × 10 9 /L. ( C ) patients with all FAB subtypes except the M3 subtype. ( D ) patients with BM blasts percentage higher than 20%. ( E ) patients with moderate cytogenetic risk stratified by cytogenetic risk. ( F ) patients with poor cytogenetic risk stratified by cytogenetic risk. ( G - I ) Time-dependent Receiver operating characteristic analysis of ARF6 in the first year, third year, and fifth year correspondingly, in which 1, 3, and 5 years were survival years

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Cox regression analysis and development of a nomogram model. Forest plot of overall survival (OS) for AML based on ( A ) univariate Cox regression analysis. ( B ) multivariate Cox regression analysis. ( C ) Nomogram model integrating ARF6 and other AML-independent prognostic factors. ( D - F ) Calibration curve of the nomogram the first year, third year, and fifth year correspondingly. In Figure D - F , each dot represents the predicted survival probability and the actual observed survival probability; each x represents the corrected result of each dot

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques:

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Functional enrichment analysis of ARF6 in AML. ( A ) Volcano diagram of differential expression of genes in high and low ARF6 expression groups in AML. ( B ) Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways conferred with ARF6 in AML. ( C ) Five up-regulated pathways identified by GSEA enrichment analysis conferred with ARF6 in AML. ( D ) Five down-regulated identified by GSEA enrichment analysis conferred with ARF6 in AML

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Functional Assay, Quantitative Proteomics, Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: ARF6 is associated with cell proliferation, adhesion, and immunomodulatory processes in AML. ( A ) Interactive analysis of GO/KEGG enrichment signal sets and corresponding genes related to cell proliferation. ( B ) Venn diagram of the gene set associated with cell proliferation. ( C ) Interactive analysis of GO/KEGG enrichment signal sets and corresponding genes related to cell adhesion. ( D ) Venn diagram of the gene set associated with cell adhesion. ( E ) Network diagram demonstrating the immune effects associated with ARF6 expression. ( F ) Demonstrate the set of genes associated with immune cell regulation of ARF6 expression. GO:0070663: regulation of leukocyte proliferation, GO:0002274: myeloid leukocyte activation, GO:0070661: leukocyte proliferation, GO:0070665: positive regulation of leukocyte proliferation, GO:0007162: negative regulation of cell adhesion, GO:0022407: regulation of cell-cell adhesion, GO:1,903,037: regulation of leukocyte cell-cell adhesion, GO:0022408: negative regulation of cell-cell adhesion, GO:0071706: tumor necrosis factor superfamily cytokine production, GO:0050670: regulation of lymphocyte proliferation, GO:0032945: negative regulation of mononuclear cell proliferation, GO:0030593: neutrophil chemotaxis, GO:0042130: negative regulation of T cell proliferation, GO:0042116: macrophage activation, GO:0050868: negative regulation of T cell activation, GO:0002367: cytokine production involved in immune response, GO:0051250: negative regulation of lymphocyte activation, GO:0042098: T cell proliferation, GO:0050863: regulation of T cell activation, GO:0002718: regulation of cytokine production involved in immune response, GO:0002443: leukocyte mediated immunity, GO:0002468: dendritic cell antigen processing and presentation, GO:0002713: negative regulation of B cell mediated immunity, GO:0021675: nerve development, GO:0045580: regulation of T cell differentiation, GO:0045582: positive regulation of T cell differentiation

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing, Activation Assay, Chemotaxis Assay, Cell Differentiation

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Association of ARF6 expression with immune cell infiltration in AML. ( A ) High ARF6 expression is associated with increased immune cell infiltration. ( B ) Connection of ARF6 expression with 11 infiltrating immune cell types. ( C ) ARF6 expression is positively correlated with multiple macrophage cell markers. ( D ) Relation between ARF6 expression and various immune cell types, including Macrophages, Neutrophils, Tem, iDC, Th17 cells, Eosinophils, NK cells, T helper cells, and T cells. * p < 0.05, ** p < 0.01, *** p < 0.001

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Protein-Protien Interaction (PPI) network analysis and hub genes. ( A ) Venn diagram shows the intersection of the top 10 hub genes obtained based on the three algorithms. ( B ) The expression levels of hub genes TLR4, ITGAX, ITGAM, FCGR3A, CD86, and CD4 in AML ( n = 173) and normal controls ( n = 70) samples. ( C ) Heatmap depicting the co-expression of ARF6 and the six hub genes. ( D - I ) Connection of ARF6 expression with each of the six hub genes. ( J - O ) Prognostic significance of six hub genes in AML. *** p < 0.001

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: Inhibition of ARF6 using NAV-2729 induces cell cycle arrest and promotes apoptosis ( A ) NAV-2729(15 µmol/l) induces cell cycle arrest in AML cell lines. ( B ) NAV-2729(15 µmol/l) promotes apoptosis in AML cell lines. * p < 0.05; ** p < 0.01

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Inhibition

Journal: Cancer Cell International

Article Title: Identification and validation of ARF6 for a potential prognostic biomarker of acute myeloid leukemia

doi: 10.1186/s12935-025-03847-2

Figure Lengend Snippet: ARF6 inhibitors regulate the PI3K/AKT/NF-κB signaling pathway to suppress the proliferation and induce apoptosis in AML cells. ( A ) Expression of BCL-2 decreased and expression of P53 and BAX increased with NAV-2729(15 μmol/l). ( B ) Expression of CCND1 and C-MYC decreased and P21 increased with NAV-2729(15 μmol/l). ( C ) The levels of PI3K/AKT/NF-κB signaling pathway-related proteins were altered by NAV-2729(15 μmol/l) treatment (n=3 in each group). (*p<0.05, **p<0.01)

Article Snippet: It is known that ARF6 influences tumor cell proliferation [ , ] and contributes to tumor-stromal cell interactions by regulating membrane trafficking and cytoskeleton remodeling, thus promoting tumor invasion and progression [ – .]

Techniques: Expressing